Simultaneous Estimation and Validation of Four Antiepileptic Drugs from Bulk and Formulations Using Reverse Phase HPLC

Epilepsy is a disorder of the central nervous system, in which nerve cell activity brain disturbed causing seizures. The objective was to develop an RP-HPLC method for consistent simultaneous quantitation four antiepileptic drugs Levetiracetam (LVT), Lamotrigine (LTG), Phenobarbital (PBT) and Phenytoin (PTY). An isocratic developed on C18 column JASCO HPLC using 5 mM potassium phosphate buffer (pH 6) acetonitrile as mobile phase at flow rate 1ml/min detected 230 nm UV detector. mean retention time LVT, LTG, PBT PTY were found 2.55, 3.55, 4.65 5.99 minutes respectively. validated per ICH guidelines be acceptable. %RSD value <2.0 % thus stating precise given range. accuracy values within 85-115% recovery specificity evaluated by assay marketed formulation, it showed percent content between 90-110% w/w all drugs. proposed analytical simple, accurate robust precisely able resolve major Hence, current can applied successfully routine examination these